Thursday 25 October 2012

Is Cancer a p53 Protein Aggregation or Prion Disease?

According to recent findings, P53 mutation can cause a conformational disease. A brazillian research team shows that p53 mutants can aggregate into prion-like amyloid oligomers and fibrils. The report states that under physiological conditions, the structure of central domain (p53c) which is made using sequence of wild type and the mutant R248Q aggregate into fibrils and amyloids.

The demonstration to prove the presence of the aggregates was done by using various techniques including electron microscopy, x-ray diffraction, FTIR, cell viability assays, dynamic light scattering and anti-amyloid immunoassays. The p53 aggregates were found in the nucleus of the tumor cell line that had p53 mutations and the seeding of a R248Q mutant with amuloid oligomers accelerated the aggregates formation. This shows that different rates of protein aggregation could explain the variability in different types of tumor cells.

We have good experience in synthesizing so many custom peptides, as most of them are large peptides. This rising sector of large peptide synthesis always uses peptides or peptide resembling compounds for pharmaceutical drug finding.

The protein p53 is vital for cell function as it suppresses tumor and regulates cellular responses to the genotoxic stresses. If there is only one functional copy of the p53 gene is present then the person is predisposed to cancer in early adulthood and generally develops independent tumors in different tissues.

We provide custom peptide and build up a proprietary synthesis strategy that lets the synthesis of peptides equal to around 120 residues-with speed and purity, usually only feasible with shorter fragments of peptides.

The p53 gene is located on the short arm of chromosome 17 and is encoded by TP53a mutation in p53 tumor suppressor is the most observed genetic alteration in human cancer. Most mutations result in a loss of DNA binding.

The p53 protein can be divided into seven domains:
1. One acidic N-terminal transcription-activation domain  or TAD, sometimes called activation domain 1 (AD1), that activates the factors of transcription.
2. An activation domain 2 (AD2) that is important for apoptotic activity.
3. A Proline rich domain also important for the apoptotic activity of p53.
4. The core DNA-binding domain (DBD) in the centre. This domain contains a single zinc atom as well as many arginine amino acids (residues 102-292). This domain is what binds the p53 co-repressor LMO3.
5. The domain that signals nuclear localization.
6. The homo-oligomerisation domain (OD). This domain is responsible for the tetramerization of the protein which is integral for p53’s activity of in vivo.
7. The C-terminal domain involved in down regulating the central DNA binding domain

The changes in p53 gene can also occur as germline mutations in some families with Li-Fraumeni syndrome. The activity of p53 can be regulated via post-translational modification such as methylation, phosphorylation and acetylation.

For more information on Bioconjugation, Custom Peptide, Peptide, Please Visit: Peptide Synthesis   




Thursday 11 October 2012

Peptide Synthesis

As most of the biologically proteins which are active and those are about 150 residues in length, though, it has been revealed that the majority of activities is kept within 100 residues.
Majority of commercial proteins are made by recombinant methods, whereby genes coding for specific sequences of peptide are inserted into a host organism, for synthesis in bulk. While the endotoxin contamination can be problematic for recombinant methods, chemical synthesis offers much cleaner products.      
We provide custom peptide and build up a proprietary synthesis strategy that lets the synthesis of peptides equal to around 120 residues-with speed and purity, usually only feasible with shorter fragments of peptides.

As most biologically those are active proteins which are around 150 residues in length, though, it has been observed that most of the activity is reserved inside 100 residues. Most proteins which are commercial are prepared by recombinant methods, whereby genes coding for specific peptide series are inserted into a host organism, for mass synthesis. At the same time as the endotoxin contamination can be difficult for those ways which are recombinant, syntheses which are chemical offers much cleaner products.     

Typical Peptide Synthesis

Our typical peptide synthesis is intended for a peptide along with average complexity. There is also available synthesis as follows:
1.    Extended peptide synthesis
2.    Alteration of peptide
3.    Library of peptide
4.    Area of nucleic acid synthesis
5.    Bioconjugation
6.    Transporter conjugations


We have good experience in synthesizing so many custom peptides, as most of them are large peptides. This rising sector of large peptide synthesis always uses peptides or peptide resembling compounds for pharmaceutical drug finding. Though, the tough challenge of long peptide synthesis is to prevail over the huge increasing result of the yield. Insufficiencies which are synthetic connected with peptide aggregation, like a sluggish or incomplete coupling or deprotection reactions, exemplify the intricacy that often arises.       

We have developed a solid-phase synthesis technology which beats these restrictions, achieving syntheses of long proteins entirely by some methods which are chemical. Our expert panel examines your favorite protein and will review possibility to make the most of the success of the synthesis from large and complicated peptides.   

For more information on Bioconjugation, Peptide, Custom Peptide, Please Visit: Peptide Synthesis   


Saturday 8 September 2012

Peptide combinations are more effective in cancer therapy

Studies says that two peptide agents used either together or single with a low-dose of a standard chemotherapy drug results more effective in cancer therapy.

Biosyn.com is a leading quality peptides and other related products manufacturing company. We are dedicated to the service of producing quality peptide and protein research. Peptides are useful in producing collagen, strengthen capillaries and increase micro circulation and increase cellular communication. Peptide improves skin firmness and elasticity and reduces the appearance of wrinkles.

Peptides play a very important role in physiological and biochemical activities of life. We use high technologies and drug manufacturing platform and serving biochemical society for several years. We are specialize in long RNA synthesis, long RNA , bioconjugations, amino acid analysis and other related products and tools.

Two or more amino acids joined in a molecular forms called peptides. The molecules are called peptides, and if the number of amino acids is less than about 50 and the longer molecules are referred to as proteins. Every living cell contains peptides which appear as enzymes, hormones, antibiotics, receptors etc.

Peptides are the great thing in anti-aging skin care. These are utilized in the most skin care products. Peptide improves skin compactness, reduces wrinkles, increases skin tonicity, increases skin firmness.

Peptide combinations delay tumor by inhibiting tumor growth and blocking the formation of new tumor blood vessels. By this way we can reduce the breast cancer and other related diseases. The short amino acid chains the HER2 peptide and VEGF peptide. The HER2 receptor molecule controls the formation of new cells and the VEGF receptor molecule plays an important role in controlling the formation of new blood vessels in tumor.

Research says that vaccinating mice with the HER2 peptide can delay the tumor growth. When this vaccination was combined with VEGF peptide, it delays tumor growth significantly. If we inject the VEGF peptide in animals, we find that tumors did not develop in 40 percent of them. By this way we can say that that VEGF peptide therapy can inhibit the formation of new cell in many cancers.

When we inject the HERF peptide vaccine it causes the immune system to generate antibodies in the body. These antibodies then bind to the over expressed HER2 receptors on caner cells, preventing them from stimulating tumor cells proliferation .

The VEGF peptide binds directly to the VEGF receptor molecules and prevents the formation of new blood vessels.

This combined therapy has not any toxic side effects. Our goal is to develop the best treatment using medicines which are not toxic.

For more information on Amino acid analysis, Oligo Synthesis, Peptide, Please Visit : Long RNA